Dna molecules encoding ligand gated ion channels from...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/12 (2006.01) C07K 14/47 (2006.01) C07K 14/705 (2006.01) C12N 5/10 (2006.01) C12N 15/63 (2006.01) C12N 15/64 (2006.01) G01N 33/53 (2006.01) G01N 33/567 (2006.01)

Patent

CA 2405473

The present invention relates in part to isolated nucleic acid molecules (polynucleotides) which encode Dermacentor variabilis ligand gated ion channel proteins. The present invention also relates to recombinant vectors and recombinant hosts which contain a DNA fragment encoding D. variabilis LGIC/GluCl channels, substantially purified forms of associated D. variabilis channel proteins and recombinant membrane fractions comprising these proteins, associated mutant proteins, and methods associated with identifying compounds which modulate associated Dermacentor variabilis LGIC/GluCl, which will be useful as insecticides and acaracides.

La présente invention concerne en partie des molécules d'acides nucléiques (polynucléotides) codant pour des protéines de canaux ioniques sensibles à des ligands de Dermacentor variabilis. La présente invention concerne également des vecteurs recombinants et des hôtes recombinants contenant un fragment d'ADN codant pour les canaux LGIC/GluCl de D. variabilis, des formes sensiblement purifiées de protéines de canaux de D. variabilis associées et des fractions de membranes recombinantes comprenant ces protéines, des protéines mutantes associées, ainsi que des méthodes associées à l'identification de composés modulant les canaux LGIC/GluCl de Dermacentor variabilis et pouvant être utilisés comme insecticides et acaricides.

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