Dna mutagenesis by random fragmentation and reassembly

Details Dna mutagenesis by random fragmentation and reassembly Dna mutagenesis by random fragmentation and reassembly

C - Chemistry, Metallurgy

12

N

C12N 15/13 (2006.01) C07H 21/00 (2006.01) C07K 16/08 (2006.01) C07K 16/12 (2006.01) C07K 16/18 (2006.01) C07K 16/28 (2006.01)

Patent

CA 2497384

A method for DNA reassembly after random fragmentation, and its application to mutagenesis of nucleic acid sequences by in vitro or in vivo recombination is described. In particular, a method for the production of nucleic acid fragments or polynucleotides encoding mutant proteins is described. The present invention also relates to a method of repeated cycles of mutagenesis, shuffling and selection which allow for the directed molecular evolution in vitro or in vivo of proteins.

L'invention porte sur une méthode permettant de ré-assembler l'ADN après une fragmentation aléatoire et sur l'application en mutagenèse de séquences d'acides nucléiques par recombinaison in vitro ou in vivo. En particulier, on décrit une méthode permettant de produire des fragments d'acide nucléiques ou des polynucléotides codant des protéines mutantes. La présente invention concerne aussi une méthode faisant appel à des cycles répétés de mutagenèse, à une recombinaison aléatoire et à la sélection, ce qui permet un processus d'évolution moléculaire dirigée des protéines in vitro ou in vivo.

Affiliated with

Also associated with

Rating

Dna mutagenesis by random fragmentation and reassembly does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Dna mutagenesis by random fragmentation and reassembly, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Dna mutagenesis by random fragmentation and reassembly will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFCA-PAI-O-1697864