Dna pcv-vaccine

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/34 (2006.01) A61K 9/00 (2006.01) A61K 39/12 (2006.01) C07K 14/01 (2006.01) C07K 14/535 (2006.01)

Patent

CA 2376523

The invention relates to immunogenic preparations or vaccines comprising, on the one hand, a plasmid encoding and expressing a gene from PCV, in particular selected from the group consisting of ORF1 of PCV-2, ORF2 of PCV-2, ORF1 of PCV-1 and ORF2 of PCV-1, and , on the other hand, an element capable of increasing the immune response directed against the product of expression of the gene, which can be a carbomer, a porcine cytokine, e.g. GM-CSF or a cationic lipid of formula (I), in which R1 is a saturated or unsaturated linear aliphatic radical having from 12 to 18 carbon atoms, R2 is another aliphatic radical comprising from 2 to 3 carbon atoms, and X is a hydroxyle or amine group. The cationic lipid can be DMRIE, possibly coupled with DOPE.

L'invention concerne des préparations ou des vaccins immunogènes comprenant, d'une part, un plasmide codant pour et exprimant un gène de PCV, en particulier sélectionné dans le groupe comprenant ORF1 de PCV-2, ORF2 de PCV-2, ORF1 de PCV-1 et ORF2 de PCV-1, et d'autre part, un élément pouvant augmenter la réponse immunitaire dirigée contre le produit de l'expression du gène, qui peut être un carbopol, une cytokine porcine, par exemple GM-CSF, ou un lipide cationique représenté par la formule (I), dans laquelle R¿1? est un radical aliphatique linéaire saturé ou non saturé comprenant de 12 à 18 atomes de carbone, R¿2? est un autre radical aliphatique comprenant de 2 à 3 atomes de carbone, et X est un groupe hydroxyle ou amine. Le lipide cationique peut être DMRIE, éventuellement couplé avec DOPE.

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