Dna polymerase blends and uses thereof

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/54 (2006.01) C12N 9/12 (2006.01) C12N 15/66 (2006.01) C12P 19/34 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2511227

The present invention discloses novel blends of chimeric and non-chimeric thermostable DNA polymerases for use in PCR, DNA sequencing and mutagenesis protocols. The invention allows for PCR reactions with shorter extension times that will facilitate PCR amplification of genomic DNA templates and improve the efficacity of long PCR.

La présente invention a trait à de nouveaux mélanges d'ADN-polymérase thermostabile chimère ou non chimère destinés à être utilisés dans la réaction en chaîne de la polymérase, le séquençage de l'ADN et les protocoles de mutagenèse. L'invention permet des temps d'extension plus courts pour les réactions en chaîne de la polymérase qui vont faciliter l'amplification PCR des matrices d'ADN et améliorer l'efficacité de la PCR longue.

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