C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/54 (2006.01) C07H 21/04 (2006.01) C12N 9/00 (2006.01) C12N 9/12 (2006.01) C12N 15/03 (2006.01) C12N 15/10 (2006.01) C12N 15/31 (2006.01) C12N 15/66 (2006.01) C12P 19/34 (2006.01) C12Q 1/68 (2006.01)
Patent
CA 2503038
The invention relates to the generation and characterization of archaeal DNA polymerase mutants with reduced base analog detection activity. The invention further provides for archaeal DNA polymerase mutants with reduced base analog detection activity containing additional mutations that modulate other DNA polymerase activities including DNA polymerization or 3'-5' exonuclease activity. The invention also discloses methods and applications of DNA polymerases with reduced base analog detection activity.
L'invention concerne la production et la caractérisation de mutants de l'ADN polymérase d'archéobactéries à activité de détection analogique à base réduite. L'invention concerne en outre des mutants de l'ADN polymérase d'archéobactéries à activité de détection analogique à base réduite contenant des mutations additionnelles qui modulent d'autres activités de l'ADN polymérase comprenant l'activité de polymérisation de l'ADN ou de 3'-5'exonucléase. L'invention concerne enfin des procédés et des applications d'ADN polymérases à activité de détection analogique à base réduite.
Ghosh Madhushree
Hogrefe Holly H.
Sorge Joseph A.
Stratagene California
Torys Llp
LandOfFree
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