C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01) C07H 21/04 (2006.01) C07K 2/00 (2006.01) C07K 5/083 (2006.01) C07K 14/575 (2006.01) C12N 15/10 (2006.01) C12P 19/34 (2006.01)
Patent
CA 2164402
DNA primers each having a nucleotide sequence substantially complementary to a nucleotide sequence which codes for Gly-Lys- Arg, Gly-Lys-Lys or Gly-Arg-Arg; methods for amplifying a polynucleotide which codes for a peptide or a precursor protein thereof using said DNA primer; screening methods for a polynucleotide which codes for a peptide comprising an amino acid sequence of Gly-Lys-Arg, Gly-Lys-Lys or Gly-Arg-Arg or a precursor protein thereof from a DNA library, etc. using said DNA; the polynucleotide obtained by said screening method; and the peptide or its precursor protein encoded by said polynucleotide, or a salt thereof. When the DNA primer of the present invention is used, the DNA which codes for a peptide having a useful physiological activity or a precursor protein thereof can be efficiently cloned.
Hosoya Masaki
Onda Haruo
Fetherstonhaugh & Co.
Takeda Chemical Industries Ltd.
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