Dna purification and recovery from high particulate and...

Details Dna purification and recovery from high particulate and... Dna purification and recovery from high particulate and...

C - Chemistry, Metallurgy

12

N

C12N 15/10 (2006.01) C07H 21/00 (2006.01) C07H 21/04 (2006.01)

Patent

CA 2484062

This invention relates to methods for rapid nucleic acid purification from sources heavily contaminated with high particulate material, such as cellular debris, and solids, including suspended solids. In particular, this invention provides methods for rapid, quantifiable recovery and purification of nucleic acids from a variety of sources heavily contaminated with solids, such as small organisms, tissue samples, samples of blood found on soil, or samples of washing from foods, which are frequently difficult sources for nucleic acid isolation due to their propensity to clog filters and columns. A device and kit are also provided.

L'invention concerne des procédés pour purifier rapidement des acides nucléiques à partir de sources fortement contaminées par une grande quantité de matériaux particulaires, tels que des débris cellulaires, et de solides, tels que des solides en suspension. L'invention concerne en particulier des procédés pour récupérer et purifier, de manière rapide et quantifiable, des acides nucléiques à partir de diverses sources fortement contaminées par des solides, tels que des organismes de petite taille, des échantillons de tissus, des échantillons de sang trouvés dans le sol, ou des échantillons provenant du lavage de denrées alimentaires, qui constituent des sources délicates pour l'isolation d'acides nucléiques en raison de leur propension à obturer les filtres et les colonnes. L'invention concerne également un dispositif et un kit correspondants.

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