Dna segment encoding a gene for a receptor related to the...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/18 (2006.01) A61K 39/395 (2006.01) A61K 47/48 (2006.01) C07K 14/71 (2006.01) C07K 14/82 (2006.01) C07K 16/28 (2006.01) C07K 16/32 (2006.01) C12N 9/12 (2006.01) C12N 15/54 (2006.01) C12Q 1/68 (2006.01) G01N 33/566 (2006.01) G01N 33/574 (2006.01) G01N 33/577 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2069900

A DNA fragment distinct from the epidermal growth factor receptor (EGF-R) and erbB-2 genes was detected by reduced stringency hybridization of v-erbB to normal genomic human DNA. Characterization of the cloned DNA fragment mapped the region of v-erbB homology to three exons with closest homology of 64 % and 67 % to a contiguous region within the tyrosine kinase domains of the EGF-R and erbB-2 proteins, respectively, cDNA cloning revealed a predicted 148 kd transmembrane polypeptide with structural features identifying it as a member of the erbB family, prompting designation of the new gene as erbB-3. It was mapped to human chromosome 12q11-13 and was shown to be expressed as 6.2 kb transcript in a variety of normal tissues of epithelial origin. Markedly elevated erbB-3 mRNA levels were demonstrated in certain human mammary tumor cell lines. These findings indicate that increased erbB-3 expression, as in the case of EGF-R and erbB-2, plays a role in some human malignancies.

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