C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01) C07H 21/04 (2006.01)
Patent
CA 2102521
2102521 9219771 PCTABS00017 The present invention provides a process for determining genotypes in highly polymorphic systems by polymerase chain reaction amplification of cDNA or genomic DNA and direct sequencing polymerase chain reaction products using oligonucleotide primers. More specifically, Class I HLA genotypes can be unambiguously determined in any subject in 16-24 hours by direct sequencing of HLA-A, HLA-B, and HLA-C transcripts enzymatically amplified and sequenced using a limited number of selected oligonucleotides.
Barbosa Jose Joaquim
Boyce-Jacino Michael Thomas
Faras Anthony James
Rich Stephen Saul
Santamaria Pedro
Goudreau Gage Dubuc
The Regents Of The University Of Minnesota
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