Dna sequence coding for a mammalian glucuronyl c5-epimerase...

C - Chemistry – Metallurgy – 12 – N

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C12N 9/90 (2006.01)

Patent

CA 2286858

An isolated or recombinant DNA sequence coding for a mammalian, including human, glucuronyl C5-epimerase or a functional derivative thereof capable of converting D-glucuronic acid (GlcA) to L-iduronic acid (IdoA); a recombinant expression vector comprising such DNA sequence; a host cell transformed with such recombinant expression vector; a process for the manufacture of a glucuronyl C5-epimerase or functional derivative thereof capable of converting GlcA to IdoA, comprising cultivation of a cell-line transformed with such recombinant expression vector; and a glucuronyl C5-epimerase or functional derivative thereof prepared by such process.

L'invention concerne une séquence d'ADN isolée ou recombinée codant pour une glucuronyle épimérase C5 mammifère, y compris, de l'homme, ou un dérivé fonctionnel de cette épimérase capable de transformer l'acide D-glucuronique (GlcA) en acide L-iduronique (IdoA). L'invention concerne également un vecteur d'expression recombiné contenant une telle séquence d'ADN; une cellule hôte transformée par un tel vecteur d'expression recombiné; un procédé de production d'une glucuronyle épimérase C5 ou d'un dérivé fonctionnel capable de transformer GlcA en IdoA, procédé consistant à mettre en culture une lignée cellulaire transformée par un tel vecteur d'expression recombiné. L'invention concerne enfin une glucuronyle épimérase C5 ou un dérivé fonctionnel préparé selon un tel procédé.

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