Dna sequence comprising a structural gene coding for xylose...

C - Chemistry – Metallurgy – 12 – N

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195/122, 195/39,

C12N 15/81 (2006.01) C12C 11/00 (2006.01) C12N 1/19 (2006.01) C12N 1/21 (2006.01) C12N 9/04 (2006.01) C12N 15/53 (2006.01) C12P 7/06 (2006.01) C12P 19/30 (2006.01)

Patent

CA 2039021

The invention relates to a DNA sequence comprising a structural gene encoding xylose reductase and/or xylitol dehydrogenase and being capable of expressing these polypeptides in a microorganism. The invention further relates to a combination of DNA sequences, a vector, a microorganism, a method for producing xylose reductase and/or xylitol dehydrogenase and the protein xylulose reductase and/or xylitol dehydrogenase. Microorganisms, expressing the structural genes comprised by the inventive DNA sequences may be used for producing ethanol from xylulose, for producing biomass and recovering NADP+ from NADPH. Preferred microorganisms are S. cerevisiae and Schizosaccharomyces pombe.

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