Dna sequence encoding plant 2-acyltransferase

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/54 (2006.01) A01H 5/00 (2006.01) A01H 5/10 (2006.01) C07K 16/40 (2006.01) C11B 1/00 (2006.01) C12N 5/10 (2006.01) C12N 9/10 (2006.01) C12N 15/11 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2212570

Plants, particularly transgenic plants, may be produced having a 2- acyltransferase enzyme from Limnanthes with an altered substrate specificity compared to the native enzyme. For example, oil seed rape Brassica napus may contain the 2-acyltransferase transgene derived from Limnanthes douglasii in order to produce trierucin. The cDNA sequence of Limnanthes douglasii 2- acyltransferase and its equivalent protein sequence are disclosed.

Il est possible de produire des plantes, et en particulier des plantes transgéniques présentant une enzyme du type 2-acyltransférase à partir de Limnanthes présentant une spécificité altérée du substrat par comparaison avec l'enzyme originale. Par exemple la graine de colza oléagineux Brassica napus peut contenir les deux transgènes de 2-acyltransférase dérivant du Limnanthes douglasii permettant de produire de la triérucine. La séquence d'ADNc de la 2-acyltransférase du Limnanthes douglasii et sa séquence protéique équivalente sont présentées ici.

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