Dna sequences encoding at least part of the tomato enzyme...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/56 (2006.01) A01H 5/00 (2006.01) C12N 5/10 (2006.01) C12N 9/24 (2006.01)

Patent

CA 2098837

2098837 9211374 PCTABS00014 The present invention provides an isolated DNA sequence which encodes at least part of the tomato enzyme endopolygalacturonase PG1 .beta.-subunit. This DNA sequence preferably encodes polypeptide the N-terminal amino acid sequence of which is: E-K-H-S-G-D-I-H-G-A-T-Y-S-D-K. In other preferred forms of the invention the DNA sequence encodes a polypeptide which includes one or both of the following amino acid sequences: D-A-N-D-I-E-A-N-T-Y-N-Y-G-Q-X-F-N-E-G; A-L-N-A-E-N-Q-V-R-I-K. The DNA sequence of the present invention may be used to produce genetically engineered tomato plants with modified riping characteristics.

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