Dna sequences encoding phytases of ruminal microorganisms

Details Dna sequences encoding phytases of ruminal microorganisms Dna sequences encoding phytases of ruminal microorganisms

C - Chemistry, Metallurgy

12

N

C12N 15/55 (2006.01) A01H 5/00 (2006.01) A23K 1/165 (2006.01) C12N 1/21 (2006.01) C12N 9/16 (2006.01) C12N 15/82 (2006.01) C12Q 1/44 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2257101

Phytases derived from ruminal microorganisms are provided. The phytases are capable of catalyzing the release of inorganic phosphorus from phytic acid. Preferred sources of phytases include Selenomonas, Prevotella, Treponema and Megasphaera. A purified and isolated DNA encoding a phytase of Selenomonas ruminantium JY35 (ATCC 55785) is provided. Recombinant expression vectors containing DNAs encoding the phytases and host cells transformed with DNAs encoding the phytases are also provided. The phytases are useful in a wide range of applications involving the dephosphorylation of phytate, including, among other things, use in animal feed supplements.

L'invention concerne des phytases dérivées de micro-organismes de ruminants. Ces phytases sont capables de catalyser le dégagement du phosphore inorganique de l'acide phytique. Les sources préférées de phytases sont Selenomonas, Prevotella, Treponema et Megasphaera. L'invention concerne également un ADN purifié et isolé codant une phytase de Selemonas ruminantium JY35 (ATCC 55785); des vecteurs d'expression de recombinaison contenant l'ADN codant les phytases et les cellules hôtes transformées par l'ADN codant les phytases. Ces phytases sont utiles dans une vaste plage d'applications telles que la déphosphorylation du phytate, applications qui comprennent, entre autre, l'utilisation des compléments alimentaires pour animaux.

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