Dna sequences, expression of said dna sequences, thermopile...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/53 (2006.01) C12N 1/15 (2006.01) C12N 9/02 (2006.01)

Patent

CA 2292723

The invention relates to DNA sequences which code for proteins with laccase activity, comprising one of the DNA sequences (SEQ ID NO:1, SEQ ID NO:2). The invention also relates to the expression of these DNA sequences, thermopile laccases coded by said DNA sequences and their use for delignifying cellulose, depolymerizing high-molecular aggregates, de-inking waste paper, polymerising aromatic compounds in waste liquids, especially waste liquids from cellulose bleaching, oxidising colorants and activating colorants to produce pigments, and their use in organic synthesis in coupling reactions of aromatic compounds or the oxidation of aromatic side chains.

L'invention concerne des séquences d'ADN qui codent pour des protéines présentant une activité de laccase et comprennent une des séquences d'ADN (SEQ ID NO:1, SEQ ID NO:2). L'invention concerne en outre l'expression de ces séquences d'ADN, les laccases thermophiles codées par ces séquences d'ADN et leur utilisation pour la délignification de la cellulose, pour la dépolymérisation d'aggrégats de haut poids moléculaire, pour le désencrage de vieux papiers, pour la polymérisation de composés aromatiques contenus dans les eaux usées, avant tout des eaux usées contenant de la lignine, résultant du blanchiment de la cellulose, pour l'oxydation de colorants ou l'activation de colorants pour la formation de pigments, ainsi que l'utilisation de ces laccases pour la synthèse organique dans les réactions de couplage de composés aromatiques ou dans l'oxydation de chaînes latérales aromatiques.

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