Dna sequencing method

Details Dna sequencing method Dna sequencing method

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) C12M 1/34 (2006.01) C12M 1/40 (2006.01) C12P 19/34 (2006.01) C12Q 1/70 (2006.01)

Patent

CA 2133956

There is disclosed a method for determining the sequence of a nucleic acid comprising the steps of: a) forming a single-stranded template comprising the nucleic acid to be sequenced; b) hybridizing a primer to the template to form a template/primer complex; c) extending the primer by the addition of a single labelled nucleotide; d) determining the type of the labelled nucleotide added onto the primer; e) removing or neutralizing the label; and f) repeating steps (c) to (e) sequentially and recording the order of incorporation of labelled nucleotides. There is also disclosed apparatus for carrying out the method.

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