Dna vaccination against cholesterol ester transfer protein...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/12 (2006.01) A61K 38/17 (2006.01) A61K 48/00 (2006.01) C07K 14/47 (2006.01)

Patent

CA 2302778

A process for inducing the production of antibodies that bind to cholesteryl ester transfer protein (CETP) is disclosed. That process comprises the steps of: (a) immunizing a mammal with an inoculum containing a recombinant DNA molecule that comprises a DNA sequence that contains (i) a sequence encoding a CETP immunogen that is linked to (ii) a promoter sequence that controls expression of the CETP immunogen, the recombinant DNA molecule being dissolved or dispersed in a vehicle; and (b) maintaining the immunized mammal for a time period sufficient to induce the production of antibodies that bind to CETP, and preferably lessen the transfer of cholesteryl esters from HDL where the blood of the mammal itself contains CETP. Immunogens, inocula, DNA segments, and recombinant DNA molecule vectors useful for carrying out the invention are also disclosed.

Procédé servant à induire la production d'anticorps se fixant à la protéine de transfert d'ester de cholestéryle (CETP). Ce procédé consiste à: (a) immuniser un mammifère au moyen d'une inoculation contenant une molécule d'ADN de recombinaison comprenant une séquence d'ADN contenant (i) une séquence codant un immunogène de CETP relié à (ii) une séquence de promoteur commandant l'expression de l'immunogène de CETP, la molécule d'ADN de recombinaison étant dissoute ou dispersée dans un véhicule; (b) maintenir le mammifère en état d'immunisation pendant une durée suffisante pour induire la production d'anticorps se fixant à CETP et diminuer, de préférence, le transfert d'esters de cholestéryle depuis HDL où le sang du mammifère lui-même contient CETP. Immunogènes, inoculums, segments d'ADN et vecteurs de molécules d'ADN de recombinaison utiles pour mettre l'invention en application.

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