C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
195/1.19, 195/1.
C12N 15/00 (2006.01) C12N 9/50 (2006.01)
Patent
CA 1272143
ABSTRACT DNA VECTORS AND THEIR USE IN RECOMBINANT DNA TECHNOLOGY. The invention provides a new class of DNA vectors, each comprising two replication systems; a first origin of replication resulting in a low copy number and stable inheritance of the plasmid, and a second, high copy number, origin of replication at which replication is directly controllable such that, when host cells carrying the vector are propagated under a first set of conditions, replication takes place mainly from the low copy number origin, and that when said cells are propa- gated under a second set of conditions, replication takes place also from the high copy number origin to produce a high yield of gene product. The controllable origin of replication may be under the control of a natural promoter of RNA transcription or a substitute promoter such as the PL promoter or lac promoter.
450724
Caulcott Celia Ann
Humphreys Gwynfor Owen
Robinson Martyn Kim
Wright Edwina Mary
Yarranton Geoffrey Thomas
Celltech Therapeutics Limited
Kirby Eades Gale Baker
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