Donor strand complemented pilin and adhesin broad-based...

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/31 (2006.01) A61K 39/108 (2006.01) A61K 39/40 (2006.01) C07K 14/245 (2006.01) C07K 16/12 (2006.01) C07K 19/00 (2006.01) C12N 15/62 (2006.01) A61K 39/00 (2006.01)

Patent

CA 2373534

Modified polypeptides containing a pilus-protein sequence and a donor complementary sequence are disclosed, as well as complexes formed of a modified polypeptide and a pilin, or pilus-protein. Also disclosed are methods of using these novel polypeptides as a means of preventing and/or treating bacterial induced diseases, especially those caused by enterobacteria such as Escherichia coli. Methods of employing these modified pilus-derived polypeptides and complexes as vaccines and for generating antibodies for further study as well as for clinical purposes are also disclosed herein. In addition, processes for large scale production of antibacterial vaccines containing said polypeptides and complexes are also described.

L'invention concerne des polypeptides modifiés contenant une séquence de protéine de pilus et une séquence complémentaire de donneur, ainsi que des complexes constitués d'un polypeptide modifié et d'une piline, ou protéine de pilus. L'invention concerne également des procédés d'utilisation de ces nouveaux polypeptides comme moyen de prévention et/ou de traitement de maladies provoquées par des bactéries, spécialement celles dues à des entérobactéries telles que Escherichia coli. L'invention concerne aussi des procédés d'utilisation de ces polypeptides et complexes dérivés de pilus comme vaccins et pour produire des anticorps en vue d'études ultérieures et à des fins cliniques. De plus, l'invention concerne des procédés de production à grande échelle de vaccins antibactériens contenant lesdits polypeptides et complexes.

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