Dose-dependent promoter originating in humans

Details Dose-dependent promoter originating in humans Dose-dependent promoter originating in humans

C - Chemistry, Metallurgy

12

N

C12N 15/09 (2006.01) C12N 1/15 (2006.01) C12N 1/19 (2006.01) C12N 1/21 (2006.01) C12N 5/00 (2006.01) C12P 21/02 (2006.01)

Patent

CA 2544300

It is found out that a novel DNA comprising the base sequence represented by SEQ ID NO:1, that has a fundamental sequence (SEQ ID NO:2), has functions as an initiator and a promoter. Thus, it is intended to provide a novel DNA comprising the base sequence represented by SEQ ID NO:1; a DNA comprising a base sequence wherein the 5~-end of the base sequence represented by SEQ ID NO:1 is added to the 3~-end of a DNA comprising one or more of the fundamental sequence (SEQ ID NO:2); the above DNA further containing a structural gene; a vector having the above DNA inserted thereinto; a transformant having the vector transferred thereinto; a method of regulating gene expression or a method of producing a protein with the use of any of the DNA, the vector and the transformant as described above; and a reagent kit containing any of the DNA, the vector and the transformant as described above.

On a découvert qu'un nouvel ADN comprenant la séquence de base représentée par SEQ ID NO:1, possédant une séquence fondamentale (SEQ ID NO 2), fonctionne comme un amorceur et un promoteur. Ainsi, l'invention concerne un nouvel ADN comprenant la séquence de base représentée par SEQ ID NO:1; un ADN comprenant une séquence de base dans laquelle l'extrémité 5' de la séquence de base représentée par SEQ ID NO:1 est ajoutée à l'extrémité 3' d'un ADN comprenant la séquence fondamentale (SEQ ID NO:2) ou plusieurs; ledit ADN contenant également un gène structural; un vecteur dans lequel ledit ADN est inséré; un transformant dans lequel le vecteur a été transféré; un procédé de régulation de l'expression génique ou un procédé de production d'une protéine à l'aide de l'ADN, du vecteur ou du transformant de l'invention; et un kit de réactif contenant l'ADN, le vecteur et le transformant de l'invention.

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