G - Physics – 01 – N
Patent
G - Physics
01
N
167/44
G01N 33/543 (2006.01) G01N 33/53 (2006.01)
Patent
CA 1110165
ABSTRACT The invention relates to quantitative or qualitative assay of an antigen in a sample, in which one brings into contact in a liquid medium, an antigen which is selected from immunogens and haptens, and is insolubilized by being carried on an insoluble support or in the form of an insoluble aggregate, a first antibody which belongs to an immunoglobulin class and which is anti- to the insolubilized antigen, and an enzyme-labelled second antibody which is anti- to the immunoglobulin class of the first antibody and which is enzyme-labelled by a covalent linkage between the enzyme and the second antibody; separates the insoluble and soluble material; and determines the enzyme activity of the soluble or insoluble separated materials, thereby determining the presence or amount of antigen in the sample. The improvement conferred by the invention lies in purifying said first antibody to remove antibodies non-specific to the insolubilized antigen, purifying the second antibody to remove antibodies non-specific to antibodies of the immuno- globulin class to which the first antibody belongs, preparing a pre-formed complex of the purified first antibody and purified first antibody and purified enzyme-labelled second antibody, and reacting the insolubilized antigen and the sample with said pre-formed complex, whereby the second antibody becomes attached to the insoluble material in inverse relationship to the amount of the antigen in the sample.
292743
Bridges James W.
Gnemmi Ernesto
Marks Vincent
Morris David
O'sullivan Michael J.
Farmitalia Carlo Erba S.p.a.
Macrae & Co.
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