Double helical oligonucleotides interfering with mrna used...

C - Chemistry – Metallurgy – 12 – N

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C12N 15/11 (2006.01) G06F 19/00 (2006.01)

Patent

CA 2640082

The present invention relates to the application of double-helical oligonucleotides (siRNA) interfering with the mRNA of gene involved in carcinogenesis, particularly the Wntl, Wnt2 or Her3 gene. Such oligonucleotides may be modified chemically, used in conjunction with viral and non-viral vectors such as lipid complexes. Such oligonucleotides exhibit unusual antiproliferative properties against tumour cells and may be used in anti-tumour treatment.

La présente invention concerne l'utilisation d'oligonucléotides à double hélice (ARNic) interférant avec l'ARNm du gène impliqué dans la carcinogenèse, notamment le gène Wntl, Wnt2 ou Her3. Ces oligonucléotides peuvent être modifiés chimiquement, utilisés en liaison avec des vecteurs viraux et non viraux comme des complexes lipidiques. Ces oligonucléotides présentent des propriétés antiprolifératives inhabituelles contre les cellules tumorales et peuvent être utilisés dans un traitement antitumoral.

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