Double selection vector

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/65 (2006.01) A01K 67/027 (2006.01) C12N 1/21 (2006.01) C12N 5/10 (2006.01) C12N 15/70 (2006.01) C12N 15/90 (2006.01)

Patent

CA 2392827

The present invention is related to a nucleic acid construct (1) to be incorporated in a double selection vector (2) able to transform a cell (3) of a specific organism, wherein- said construct (1) contains two different genes (10 and 11), each gene encoding a different toxic molecule (4 and 5) to a prokaryote cell, preferably to E. Coli, said genes (10 and 11) being disposed upstream and downstream a cassette sequence (8), or downstream and upstream site(s) for the insertion of a cassette sequence (8), and- said nucleic acid construct comprises specific sequence portions (12, 12') allowing inactivation of said genes (10 and 11).

L'invention concerne une construction d'acide nucléique (1) qui s'incorpore dans un vecteur de double sélection (2) pouvant transformer une cellule (3) d'un organisme spécifique. Ladite construction (1) contient deux gènes différents (10 et 11), chaque gène codant une molécule toxique différente (4 et 5) pour une cellule procaryote, de préférence E. coli. Ces gènes (10 et 11) sont disposés en amont et en aval d'une séquence de cassette (8), ou en aval et en amont d'un site ou de sites pour l'insertion d'une séquence de cassette (8). La construction d'acide nucléique comprend des parties de séquence spécifiques (12, 12') qui permettent l'inactivation desdits gènes (10 et 11).

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