Double-stranded hairpin rnas for rnai

C - Chemistry – Metallurgy – 12 – N

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C12N 15/11 (2006.01)

Patent

CA 2527907

The invention is directed towards constructs for RNAi techniques. The invention provides a ribonucleic acid (RNA) for use as interfering RNA in gene silencing techniques to silence a target gene comprising in a 5' to 3' direction at least a first effector sequence, a second effector sequence, a sequence substantially complementary to the second effector sequence and a sequence substantially complementary to the first effector sequence, wherein the complementary sequences are capable of forming double stranded regions with their respective effector sequences and wherein at least one of these sequences is substantially identical to the predicted transcript of a region of the target gene, and a nucleic acid construct encoding such an RNA.

La présente invention concerne des produits de recombinaison destinés aux techniques d'interférence à l'ARN. L'invention se rapporte à un acide ribonucléique (ARN) destiné à être utilisé comme ARN d'interférence dans les techniques d'extinction génique afin d'entraîner l'extinction d'un gène cible comprenant dans une direction de 5' à 3' au moins une première séquence effectrice, une seconde séquence effectrice, une séquence sensiblement complémentaire de la seconde séquence effectrice et une séquence sensiblement complémentaire de la première séquence effectrice, les séquences complémentaires étant capables de former des régions double brin avec leurs séquences effectrices respectives et l'une de ces séquences au moins étant sensiblement identique au transcrit prédit d'une région du gène cible. L'invention se rapporte également à un acide nucléique de recombinaison codant l'ARN précité.

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