Double transposition methods for manipulating nucleic acids

Details Double transposition methods for manipulating nucleic acids Double transposition methods for manipulating nucleic acids

C - Chemistry, Metallurgy

12

N

C12N 15/90 (2006.01) C12N 9/22 (2006.01) C12N 15/10 (2006.01) C12N 15/63 (2006.01)

Patent

CA 2431246

Methods are provided for manipulating nucleic acid to produce gene fusions, to delete or clone a portion of a chromosome, or to insert a sequence into a chromosome. The methods employ sequential transposition processes using two or more pairs of inverted repeat transposase-interacting sequences on a transposable polynucleotide wherein each pair of transposase-interacting sequences interacts with a distinct transposase enzyme.

L'invention concerne des procédés permettant de manipuler des acides nucléiques en vue de produire des fusions géniques, d'effacer ou de cloner une partie d'un chromosome ou d'insérer une séquence dans un chromosome. Les procédés mettent en oeuvre des procédés de transposition séquentielle au moyen de deux ou plusieurs séquences inversées de répétition interagissant avec une transposase sur un polynucléotide transposable, chaque paire de séquences interagissant avec une transposase interagissant avec une enzyme transposase distincte.

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