Dp and e2f protein nuclear localisation signals and their use

Details Dp and e2f protein nuclear localisation signals and their use Dp and e2f protein nuclear localisation signals and their use

C - Chemistry, Metallurgy

07

K

C07K 19/00 (2006.01) C07K 14/47 (2006.01) C12N 15/67 (2006.01) C12Q 1/02 (2006.01) C12Q 1/68 (2006.01) G01N 33/53 (2006.01)

Patent

CA 2253838

The present invention provides nuclear localisation signals derived from the DP-3 and E2F-1 transcription factors and the use of these signals in assays for regulators of cell cycle progression. Such assays involve using the signals to direct a marker gene to the nucleus and determining whether the nuclear localisation of the marker is disrupted by the presence of a putative regulator.

La présente invention concerne des signaux de localisation nucléaire issus des facteurs de transcription DP-3 et E2F-1 ainsi que l'utilisation de ces signaux dans des dosages de régulateurs de l'évolution du cycle cellulaire. Un tel dosage fait intervenir l'utilisation des signaux pour diriger un gène marqueur vers le noyau et pour déterminer si la localisation du marqueur est interrompue par la présence d'un régulateur putatif.

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