Dry analytical element for acetaminophen assay

Details Dry analytical element for acetaminophen assay Dry analytical element for acetaminophen assay

C - Chemistry, Metallurgy

12

Q

C12Q 1/34 (2006.01)

Patent

CA 2099639

A spectrophotometric assay for the detection of acetaminophen in aqueous fluids can be carried out with a dry analytical element. The element comprises a support having thereon one or more reagent layers containing a first enzyme, aryl acylamidase, to cleave the amide bond of acetaminophen to produce p-aminophenol; a second enzyme, ascorbic acid oxidase, to oxidize the p-aminophenol so that it couples to a water soluble coupling agent to form a dye that is read at 670nm. The assay is precise, accurate on serum and plasma samples, and relatively free from significant interferences. The element also allows measurement over a broad dynamic range.

Il est possible de réaliser, avec un élément analytique sec, un dosage spectrophotométrique de l'acétaminophène dans les fluides aqueux. Cet élément comprend un support composé d'une ou de plusieurs couches de réactifs contenant une première enzyme, l'aryl acylamidase, pour couper la liaison amide de l'acétaminophène afin de produire du p-aminophénol; une seconde enzyme, l'ascorbate oxydase, pour oxyder le p-aminophénol afin qu'il se lie à un agent de couplage hydrosoluble et forme un colorant lisible à 670 nm. Le dosage dans les échantillons de sérum et de plasma est précis, exact et relativement exempt d'interférences significatives. L'élément permet également d'effectuer des mesures dans un vaste intervalle.

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