Dry analytical element for assaying salicylate

Details Dry analytical element for assaying salicylate Dry analytical element for assaying salicylate

C - Chemistry, Metallurgy

12

Q

150/15.1

C12Q 1/26 (2006.01) G01N 33/52 (2006.01)

Patent

CA 2022979

A dry multilayer element for assaying salicylate is disclosed. The element comprises a) a spreading layer, b) a dye layer comprising tyrosinase and a hydrazone coupling agent, c) a reagent layer comprising salicylate hydroxylase and nicotinamide adenine dinucleotide (NADH), characterized in that d) the dye layer has a pH greater than 6.5; e) the dye layer and reagent layer are separated by a barrier layer that prevents passage of molecules having a molecular weight in excess of 5,000, and f) the hydrazone coupling agent is water insoluble at a pH above 6.5.

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