C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/60 (2006.01) C12Q 1/26 (2006.01) C12Q 1/28 (2006.01) C12Q 1/34 (2006.01) G01N 33/92 (2006.01)
Patent
CA 2474843
A dry analytical element is disclosed which can be used for the quantification of high-density lipoprotein cholesterol (HDLC). The element comprises a support having one or more reagent layers containing a first enzyme, a cholesterol ester hydrolase, to hydrolyze cholesterol esters, a second enzyme, cholesterol oxidase, to release hydrogen peroxide from cholesterol, and a third enzyme, horseradish peroxidase, to oxidize a leuco dye that is read at 670 nm. The element also contains phosphotungstic acid, a non-high-density lipoprotein precipitant, and a high-density lipoprotein selective surfactant, which together confer HDLC selectivity on the assay. Also disclosed are polymers that improve assay precision and eliminate interference from hemolyzed patient samples.
Arter Thomas Charles
Chambers Deborah Lynn
Dimagno Theodore John
Silva David Paul Jr.
Vavra Karen J.
Norton Rose Canada S.e.n.c.r.l.,s.r.l./llp
Ortho-Clinical Diagnostics Inc.
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