C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 5/00 (2006.01) C12N 5/07 (2010.01) C12N 1/00 (2006.01) C12N 5/02 (2006.01)
Patent
CA 2427596
The present invention relates generally to nutritive (e.g., cell culture) medium, medium supplement, media subgroup and buffer formulations. Specifically, the present invention provides powdered nutritive medium, medium supplement and medium subgroup formulations, particularly cell culture medium supplements (including powdered sera such as powdered fetal bovine seum (FBS)), medium subgroup formulations and cell culture media comprising necessary nutritive factors that facilitate the in vitro cultivation of cells. The invention further provides such powdered formulations that produce particular or desired final ionic and/or pH conditions upon reconstitution with a solvent. The invention si also directed to methods of roduction of these formulations, and also provides kits and methods for cultivation of prokaryotic and eukaryotic cells using these formulations. The invention also relates to methods of producing sterile formulations, and to methods for producing dry cell powders. The invention also provides cell, media, media supplement, media subgroup and buffer powders produced by the methods of the invention.
L'invention concerne généralement des formulations de milieu nutritif (par exemple, de culture cellulaire), de complément de milieu, de sous-groupes de milieu, et de tampons. L'invention concerne, plus spécifiquement, des formulations en poudre de milieu nutritif, de complément de milieu, et de sous-groupes de milieu, notamment des compléments de milieu de culture cellulaire (y compris du sérum en poudre tel que du sérum de foetus de bovin (FBS)), des formulations de sous-groupes de milieu, et des milieux de culture cellulaire comprenant les facteurs nutritifs nécessaires qui facilitent la culture in vitro de cellules. L'invention concerne également des formulations en poudre présentant des conditions ioniques et de pH finales particulières ou désirées après reconstitution avec un solvant. L'invention concerne, en outre, des méthodes de production de ces formulations, et des trousses et méthodes de culture de cellules procaryotes et eucaryotes à l'aide desdites formulations. L'invention concerne également des méthodes de production de formulations stériles, et des méthodes de production de poudres de cellules sèches. L'invention concerne enfin des poudres de cellules, de milieux, de compléments de milieu, de sous-groupes de milieu, et de tampons produites grâce à ces méthodes.
Dadey Barbara Mary
Fike Richard Milton
Hassett Richard Francis
Radominski Robert Chester
Invitrogen Corporation
Life Technologies Corporation
Mbm Intellectual Property Law Llp
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