Dsrna as insect control agent

Details Dsrna as insect control agent Dsrna as insect control agent

C - Chemistry, Metallurgy

12

N

C12N 15/82 (2006.01) A01H 5/00 (2006.01) C12N 5/10 (2006.01) C12N 15/11 (2006.01) C12N 15/12 (2006.01)

Patent

CA 2627795

The present invention concerns methods for controlling insect infestation via RNAi- mediated gene silencing, whereby the intact insect cell(s) are contacted with a double-stranded RNA from outside the insect cell(s) and whereby the double-stranded RNA is taken up by the intact insect cell(s). In one particular embodiment, the methods of the invention are used to alleviate plants from insect pests. Alternatively, the methods are used for treating and/or preventing insect infestation on a substrate or a subject in need of such treatment and/or prevention. Suitable insect target genes and fragments thereof, dsRNA constructs, recombinant constructs and compositions are disclosed.

L'invention concerne des procédés destinés à lutter contre l'infestation d'insectes par silençage génique induit par ARNi, la/les cellule(s) d'insecte intacte(s) étant mise(s) en contact avec un ARN bicaténaire de l'extérieur de la/des cellule(s) d'insecte, l'ARN bicaténaire étant absorbé par la/les cellule(s) d'insecte intacte(s). Dans un mode de réalisation particulier, les procédés de l'invention sont utilisés afin d'alléger les plantes d'insectes ravageurs. Dans un autre mode de réalisation, les procédés sont utilisés afin de traiter et/ou prévenir l'infestation d'insectes sur un substrat ou un sujet nécessitant un tel traitement et/ou une telle prévention. L'invention concerne également des gènes cibles d'insectes appropriés et des fragments de ceux-ci, des constructions d'ARNds, des constructions et des compositions recombinantes.

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