Dual affinity polypeptides for purification

Details Dual affinity polypeptides for purification Dual affinity polypeptides for purification

C - Chemistry, Metallurgy

07

K

C07K 1/22 (2006.01)

Patent

CA 2705334

The present invention relates to a process for purification of a target biomolecule, comprising the steps: (a) contact-ing (i) a target biomolecule, (ii) a dual affinity polypeptide, and (iii) a solid support comprising a catching ligand, wherein the ratio between the equilibrium dissociation constants of the dual affinity polypeptide, [K D,t / K D,s], is at least 10° at standard conditions; and (b) recovering the target biomolecule by elution.

La présente invention concerne un procédé de purification d'une biomolécule cible qui comprend les étapes consistant à : (a) mettre en contact (i) une biomolécule cible, (ii) un polypeptide à double affinité et (iii) un support solide comportant un ligand de capture, ledit rapport entre les constantes de dissociation à l'équilibre du polypeptide à double affinité, [KD, t / KD, s ], valant au moins 10° dans les conditions standards ; et (b) récupérer la biomolécule cible par élution.

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