Dual purpose tissue fixative

Details Dual purpose tissue fixative Dual purpose tissue fixative

G - Physics

01

N

G01N 1/30 (2006.01) G01N 1/28 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2147593

A dual purpose tissue fixative is described. The fixative comprises a cross-linking aldehyde, alcohol, a chelating agent and a non-amine buffer. More particularly, the tissue fixative comprises 0.1-2 % w/v formaldehyde, 45-90% w/w alcohol, 1-10 mM of a chelating agent and 1-50 mM of a non-nitrogen buffer. The tissue fixative permits the recovery of high molecular weight DNA and RNA from the tissue sample for molecular genetic analysis. The tissue fixative also preserves the morphology and immunogenicity of the tissue allowing for pathology analysis. The tissue fixative is compared to 95% alcohol and a standard fixative such as 10% BNF.

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