Dye-providing composition, diagnostic test kit and their use...

Details Dye-providing composition, diagnostic test kit and their use... Dye-providing composition, diagnostic test kit and their use...

C - Chemistry, Metallurgy

12

Q

8/45, 167/45, 15

C12Q 1/28 (2006.01) G01N 33/76 (2006.01)

Patent

CA 1321045

-0- DYE-PROVIDING COMPOSITION, DIAGNOSTIC TEST KIT AND THEIR USE IN METHOD FOR LIGAND DETERMINATION USING PEROXIDASE LABELED-RECEPTOR Abstract of the Disclosure A dye-providing composition comprises a water-soluble or -dispersible polymer, such as a vinylpyrrolidone polymer, and an imidazole leuco dye capable of providing a dye in the presence of hydro- gen peroxide and a peroxidative substance. The weight ratio of polymer to leuco dye is from about 10,000:1 to about 100:1. The dye-providing composi- tion can be included with a peroxidase substrate in a diagnostic test kit. A method for the determination of a ligand can be carried out using a peroxidase labeled-receptor for the ligand and the dye-providing composition described above. The method is particu- larly useful for the determination of human chorionic gonadotropin (hCG).

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