C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 1/68 (2006.01) C07D 221/12 (2006.01) C07D 417/06 (2006.01) C07H 21/00 (2006.01) C09B 23/04 (2006.01) C09B 57/00 (2006.01)
Patent
CA 2162710
Novel fluorescent dyes are provided, characterized by having a fluorophore joined to a polycationic chain of at least two positive charges. The dyes are found to provide for high enhancement upon binding to nucleic acid and have strong binding affinities to the nucleic acid, as compared to the fluorophore without the polycationic chain. The dyes find use in detection of dsDNA in gel electrophoresis and solution at substantially higher sensitivities using substantially less dye.
Benson Scott C.
Glazer Alexander N.
Fetherstonhaugh & Co.
The Regents Of The University Of California
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