E.coli o157:h7 c1-1nh-binding protein and methods of use

Details E.coli o157:h7 c1-1nh-binding protein and methods of use E.coli o157:h7 c1-1nh-binding protein and methods of use

C - Chemistry, Metallurgy

12

N

C12N 9/52 (2006.01) A61K 38/48 (2006.01) A61K 38/57 (2006.01) A61K 39/00 (2006.01) A61K 39/40 (2006.01) C07K 16/40 (2006.01) C12N 15/31 (2006.01) C12Q 1/37 (2006.01) C07K 16/12 (2006.01)

Patent

CA 2555251

Disclosed is a pO157 plasmid-specified polypeptide found in E. Coli EDL933 and other E. Coli that binds to and cleaves C1-esterase inhibitor, and antibodies specific for the polypeptide. Also disclosed are methods employing the polypeptide for diagnosing enterohemorrhagic E. Coli infection, identifying potential inhibitors of its activity, and reducing viscosity of material containing glycosylated polypeptides.

Un polypeptide spécifié à plasmide pO157 trouvé dans <i>E. Coli </i>EDL933 et un autre <i>E. Coli</i> se liant à et clivant un inhibiteur de C1-estérase, ainsi que des anticorps spécifiques du polypeptide. Des procédés d'utilisation du polypeptide pour le diagnostic de l'infection antérohémorragique de <i>E. Coli</i>, l'identification d'inhibiteurs éventuels de son activité et la réduction de la viscosité d'une matière contenant des polypeptides glycosylés.

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