C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 9/24 (2006.01) C12N 9/26 (2006.01) C12N 9/42 (2006.01)
Patent
CA 2372594
The invention provides a method for making a glycosyl hydrolase characterized by an increase in catalytic activity on an insoluble substrate, comprising replacing an active site associated glycosyl-stabilizing amino acid of the hydrolase with an amino acid, the replacing amino acid not strongly binding a disaccharide product in the active site, and a method for making a glycosyl hydrolase characterized by an increase in catalytic activity on a soluble substrate, comprising replacing a hydrophobic substrate binding amino acid of the hydrolase with a positively charged amino acid. The invention further provides a glycosyl hydrolase, comprising Y42R (SEQ. ID NO:1), W82R (SEQ. ID NO:2), or Y245G (1) (SEQ. ID NO:3) and the DNA sequences encoding the enzymes.
L'invention concerne un procédé de fabrication d'une glycosyl hydrolase caractérisée par une activité catalytique accrue sur un substrat insoluble. Le procédé consiste à remplacer un acide aminé glycosyl-stabilisant associé au site actif de l'hydrolase par un acide aminé, l'acide aminé de remplacement ne se liant pas fortement à un produit de disaccharide au site actif. L'invention concerne aussi un procédé de fabrication d'une glycosyl hydrolase caractérisée par une activité catalytique accrue sur un substrat soluble, qui consiste à remplacer un acide aminé de l'hydrolase se liant au substrat hydrophobe par un acide aminé chargé positivement. L'invention a en outre trait à une glycosyl hydrolase contenant Y42R (SEQ. ID NO:1), W82R (SEQ. ID NO:2), ou Y245G (1) (SEQ. ID NO:3) et aux séquences d'ADN codant pour ces enzymes.
Adney William S.
Baker John O.
Decker Stephen R.
Himmel Michael E.
Sakon Joshua
Finlayson & Singlehurst
Midwest Research Institute
LandOfFree
E1 endoglucanase variants y245g, y82r and w42r does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with E1 endoglucanase variants y245g, y82r and w42r, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and E1 endoglucanase variants y245g, y82r and w42r will most certainly appreciate the feedback.
Profile ID: LFCA-PAI-O-1366150