Early diagnosis of sepsis utilizing antigen-antibody...

Details Early diagnosis of sepsis utilizing antigen-antibody... Early diagnosis of sepsis utilizing antigen-antibody...

G - Physics

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G01N 33/569 (2006.01) G01N 1/38 (2006.01) G01N 21/76 (2006.01) G01N 33/50 (2006.01) G01N 33/52 (2006.01) G01N 33/577 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2097952

A method for determining the extent of sepsis in a patient by detecting the presence of antigen indicative of sepsis in a patient blood-derived test sample containing white blood cell fractions, the method comprises: i) incubating the test sample with antibodies specific to the antigens indicative of sepsis to form antibody/antigen complexes, ii) introducing a chemiluminescent compound to the test sample, and iii) allowing the antibody/antigen complexes to interact with the white blood cell fractions to produce oxidants. After the above steps are conducted in any order then: iv) the oxidants reacting with the chemiluminescent compounds cause luminescent light to be emitted from the test sample, and v) the emitted light over a predetermined period is measured and correlated with the extent of sepsis.

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