Early diagnostic test

C - Chemistry – Metallurgy – 12 – Q

Patent

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Details

C12Q 1/04 (2006.01) G01N 33/53 (2006.01) G01N 33/569 (2006.01) G01N 33/58 (2006.01)

Patent

CA 2222886

A method for screening for Lyme borreliosis and other infectious diseases early in the course of the disease are disclosed. It has been found that there is a dramatic difference in surface binding of cultured infectious agents to naturally occurring polymorphonuclear leukocytes (PMNs) (e.g., neutrophils) between infected and noninfected animals, which may be present early in the course of the disease, before measurable antibody response. This binding response can be rapidly and accurately distinguished and quantitated using various detection techniques. The method involves visualizing the surface binding of the cultured infectious agent to naturally-occurring PMN to detect the infection.

Le procédé décrit permet de dépister de manière précoce la maladie de Lyme et d'autres maladies infectieuses au cours de l'évolution de la maladie. On a découvert une différence frappante dans le degré de liaison superficielle d'agents infectieux cultivés à des leucocytes polynucléaires naturels (par exemple, des neutrophiles) chez des animaux infectés et non infectés. Cette différence peut être présente tôt dans l'évolution de la maladie, avant toute réponse mesurable des anticorps. Cette réaction de liaison peut être détectée et quantifiée rapidement et avec précision au moyen de diverses techniques de détection. Selon ce procédé, on visualise la liaison superficielle des agents infectieux cultivés aux leucocytes polynucléaires naturels afin de détecter l'infection.

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