Early mesoderm cells, a stable population of mesendoderm...

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 5/073 (2010.01) C12N 5/071 (2010.01) C12N 5/0735 (2010.01) C12N 5/0775 (2010.01) A61K 35/12 (2006.01) A61K 35/34 (2006.01) C12N 1/04 (2006.01)

Patent

CA 2676044

The present invention relates to the production of multipotent migratory cell (MMCs) which can be differentiated into mesoderm or endoderm lineages. Multipotent Migratory Cells (MMC) are stable and robust and can be passaged at least 20 times (perhaps indefinitely), can be recovered after freezing, reamplified and differentiated into multiple lineages. They are therefore storage stable. The method of producing these cells points to a way to generate a multipotent cell type (mesendoderm) from blastocycts for the generation of therapeutically useful cell types without going through a classical hESC state. The production of multipotent migratory cells, mesendoderm cells and mesoderm cells (IsIl+) is also described.

La présente invention concerne la production de cellules migratoires multipotentes (MMC) qui peuvent être différenciées dans les lignées mésodermiques ou endodermiques. Les cellules migratoires multipotentes (MMC) sont stables et robustes et peuvent être transférées au moins 20 fois (peut-être indéfiniment), peuvent être récupérées après congélation, réamplifiées et différenciées dans de multiples lignées. Elles sont par conséquent stables au stockage. Le procédé de production de ces cellules montre une façon de générer un type de cellules multipotentes (mésendodermique) à partir de blastocystes pour la génération de type de cellules thérapeutiquement utiles sans passer par un état de hESC classique. La production de cellules migratoires multipotentes, de cellules mésendodermiques et de cellules mésodermiques (IsIl+) est également décrite.

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