Ectocornea-like sheet and method of constructing the same

A - Human Necessities – 61 – L

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A61L 27/38 (2006.01) C12N 5/071 (2010.01) A61L 27/36 (2006.01) A61F 2/14 (2006.01)

Patent

CA 2466903

It is intended to provide a transplantation material applicable to eye-surface diseases with a need for ectocornea transplantation (i.e., an ectocornea-like sheet). Oral mucosal epithelial cells are inoculated onto an amnion and then cultured in the coexistence of supporter cells. When a layered structure of the oral mucosal epithelial cells is formed, the outermost layer is brought into contact with air, thereby inducing differentiation. Thus, an ectocornea- like sheet having an oral mucosal epithelial cell layer on the amnion is obtained.

L'invention concerne un matériau de transplantation destiné au traitement des maladies de la surface de l'oeil requérant une transplantation de l'épithélium cornéen (c'est-à-dire, une feuille du type épithélium cornéen). Des cellules épithéliales de la muqueuse orale sont inoculées à une membrane amniotique, puis cultivées en présence de cellules de support. Lorsqu'une structure en couches des cellules épithéliales de la muqueuse orale est formée, la couche la plus extérieure est mise en contact avec l'air, ce qui induit une différenciation. On obtient ainsi une feuille du type épithélium cornéen comportant une couche de cellules épithéliales de la muqueuse orale.

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