Editing-based selectable plastid marker genes

Details Editing-based selectable plastid marker genes Editing-based selectable plastid marker genes

C - Chemistry, Metallurgy

12

N

C12N 15/82 (2006.01) C07K 14/415 (2006.01) C12N 15/29 (2006.01) C12N 15/62 (2006.01) C12N 15/65 (2006.01)

Patent

CA 2257867

Disclosed are novel DNA constructs for selecting plastid transformants in higher plants. Also disclosed are editing based selectable marker genes which require editing at the transcriptional level for expression of the selectable marker gene. Vectors including such edited upstream sequences operably linked to slectable marker genes facilitate the isolation of plastid, rather than nuclear transformants in higher plants.

L'invention concerne de nouvelles constructions d'ADN permettant de sélectionner des transformants plastidiaux chez des plantes supérieures. L'invention concerne également des gènes marqueurs sélectionnables fondés sur l'édition, ladite édition devant s'effectuer au niveau de la transcription pour l'expression du gène marqueur sélectionnable. Des vecteurs, y compris lesdites séquences amont éditées et liées fonctionnellement à des gènes marqueurs sélectionnables, facilitent, chez des plantes supérieures, l'isolement de transformants plastidiaux plutôt que de transformants nucléaires.

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