Effective polyionic charged member on a label in immunoassay

Details Effective polyionic charged member on a label in immunoassay Effective polyionic charged member on a label in immunoassay

G - Physics

01

N

167/45

G01N 33/58 (2006.01) C12Q 1/00 (2006.01) G01N 33/542 (2006.01)

Patent

CA 1136040

ABSTRACT OF THE DISCLOSURE A method for determining a member of a specific binding pair-ligand and receptor (antiligand). Reagents employed include a first modified member which provides an electrical field due to the presence of a plurality of ionic charges and a second modified member labeled with a component of a signal producing system, which system may have one or more components. The average proximity in the assay medium of the first and second modified members is related to the amount of analyte, where the observed signal from the signal producing system is related to the effect of the electrical field on the signal producing system. Also, compositions are provided, as well as reagents, in predetermined ratios for optimizing the signal response to variations in analyte concentration.

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