Efficient and stable in vivo gene transfer to cardiomyocytes...

A - Human Necessities – 61 – K

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A61K 48/00 (2006.01) A61P 9/00 (2006.01) C12N 15/864 (2006.01)

Patent

CA 2356551

This invention relates to the use of recombinant adeno-associated virus (rAAV) vectors to transduce cardiomyocytes $I(in vivo) by infusing the rAAV into a coronary artery or coronary sinus. rAAV infection is not associated with detectable myocardial inflammation or myocyte necrosis. Thus, rAAV is a useful vector for the stable expression of therapeutic genes in the myocardium and can be used to deliver genes for inducing angiogenesis, inhibiting angiogenesis, stimulating cell proliferation, inhibiting cell proliferation and/or treating or ameliorating other cardiovascular conditions.

L'invention concerne l'utilisation de vecteurs viraux adéno-associés de recombinaison (rAAV) pour la transduction de cardiomyocytes in vivo par injection de vecteurs rAAV dans une artère ou un sinus coronaire. L'infection au virus rAAV n'est pas associée à une inflammation du myocarde ou à la nécrose des myocytes détectable. Ainsi, le vecteur rAAV est un vecteur utile pour l'expression stable de gènes thérapeutiques dans le myocarde, et il peut être utilisé pour un apport de gènes destiné à induire l'angiogenèse, à inhiber l'angiogenèse, à stimuler la prolifération cellulaire, à inhiber la prolifération cellulaire et/ou à traiter ou à soulager d'autres troubles cardiovasculaires.

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