Efficient construction of gene targeting vectors

Details Efficient construction of gene targeting vectors Efficient construction of gene targeting vectors

C - Chemistry, Metallurgy

12

N

C12N 15/90 (2006.01) A01K 67/027 (2006.01) C12N 15/10 (2006.01) C12N 15/70 (2006.01) C12N 15/81 (2006.01) C12N 15/85 (2006.01)

Patent

CA 2308016

The present invention is directed to methods for producing or obtaining gene targeting constructs by way of homologous recombination in host cells and to targeting constructs produced by those methods. The invention is also directed to transgenic animals having targeted mutations introduced in to the cells of the animal using the targeting constructs of the invention.

Cette invention se rapporte à des procédés servant à fabriquer ou à obtenir des produits de synthèse de ciblage génétique par recombinaison homologue dans des cellules hôtes, ainsi qu'aux produits de synthèse de ciblage fabriqués par ces procédés. Cette invention concerne également des animaux transgéniques comportant des mutations ciblées, qui ont été introduites dans les cellules de l'animal au moyen des produits de synthèse de ciblage faisant l'objet de cette invention.

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