Efficient ex vivo expansion of cd4+ and cd8+ t-cells from...

C - Chemistry – Metallurgy – 12 – N

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C12N 5/0783 (2010.01) A61K 35/14 (2006.01) C12N 5/10 (2006.01)

Patent

CA 2326378

Methods for the expansion of CD4, CD8, and DP T-cells from HIV infected patients are disclosed which allow the maintenance of low levels of HIV. The invention further discloses methods for the inhibition of HIV gene expression. Also disclosed are methods for the rapid and efficient screening of cells derived from HIV-infected patients to assess the suitability of various antiviral treatments. The invention further provides a means for the generation of cell banks for use in immune reconstitution and means of treating or modifying expanded cell populations prior to infusion to enhance or modulate therapeutic effectiveness.

L'invention concerne des méthodes de développement de lymphocytes T à CD4, CD8 et DP prélevés sur des patients infectés par le VIH, permettant de maintenir le VIH à des niveaux réduits. L'invention concerne également des méthodes d'inhibition du développement de gènes à VIH. De plus, l'invention concerne des méthodes de criblage rapide et efficace de cellules prélevées sur des patients infectés par le VIH afin d'évaluer les divers traitements antiviraux appropriés. En outre, l'invention concerne un moyen de production de banques de cellules destinées à être utilisées dans une reconstitution immunitaire ainsi qu'un moyen de traitement ou de modification de populations de cellules développées avant perfusion afin d'améliorer ou de moduler l'efficacité thérapeutique.

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