Efficient gene silencing in plants using short dsrna sequences

Details Efficient gene silencing in plants using short dsrna sequences Efficient gene silencing in plants using short dsrna sequences

A - Human Necessities

01

H

A01H 1/00 (2006.01) C12N 15/11 (2006.01) C12N 15/82 (2006.01)

Patent

CA 2516310

Methods and means are provided to increase the efficiency of gene silencing when using dsRNA sequences which have a stem length shorther than about 200 basepairs by providing chimeric genes encoding such dsRNA sequences with a promoter recognized by DNA dependent RNA polymerase III comprising all cis- acting promoter elements which interact with DNA dependent RNA polymerase III.

L~invention concerne des procédés et des moyens pour accroître l~efficacité du blocage génique lors de l~utilisation de séquences d~ARN bicaténaire présentant une longueur de tige inférieure à environ 200 paires de bases. A cet effet, des gênes chimères codant pour ces séquences d~ARN bicaténaire sont dotés d~un promoteur reconnu par l~ARN-polymérase III ADN-dépendante. Ce promoteur comporte tous les éléments à action en cis qui entrent en interaction avec l~ARN-polymérase III ADN-dépendante.

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