Efficient haploid cell sorting for flow cytometer systems

G - Physics – 01 – N

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G01N 33/00 (2006.01)

Patent

CA 2566749

A flow cytometry system (1) for sorting haploid cells, specifically irradiatable sperm cells, with an intermittingly punctuated radiation emitter (56). Embodiments include a beam manipulator (21) and even split radiation beams directed to multiple nozzles (5). Differentiation of sperm characteristics with increased resolution may efficiently allow differentiated sperm cells to be separated higher speeds and even into subpopulations having higher purity.

L'invention concerne un système de cytométrie en flux (1) permettant de trier des cellules haploïdes, de manière spécifique des cellules spermatiques pouvant être irradiées, au moyen d'un émetteur de rayonnement à émission intermittente (56). Des modes de réalisation de l'invention font intervenir un manipulateur (21) de faisceau et des faisceaux de rayonnement divisés dirigés vers plusieurs ajutages (5). La différenciation des caractéristiques du sperme à résolution améliorée permet de séparer de manière efficace les cellules spermatiques différenciées à des vitesses supérieures et également en sous-populations de pureté supérieure.

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