Efficient liposomal encapsulation

A - Human Necessities – 61 – K

Patent

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Details

A61K 9/127 (2006.01) B01J 13/02 (2006.01)

Patent

CA 2471960

The present invention concerns a method for preparing liposomes, said method comprising the following steps: (I) mixing at least one liposome-forming lipid, a water-miscible organic solvent and aqueous medium Y to form a gel or liquid containing gel particles without sonication; and thereafter (II) (a) mixing the gel or liquid containing gel particles with aqueous medium Z1 to directly form the liposomes; (b) (i) mixing the gel or liquid containing gel particles with aqueous medium Z1 to form a curd or curdy substance; and (ii) mixing the curd or curdy substance with aqueous medium Z2 to directly form the liposomes; or (c) (i) cooling the gel or liquid containing gel particles to form a waxy substance; and (ii) mixing the waxy substance with aqueous medium Z1 to directly form the liposomes; wherein aqueous media Y, Z1 and Z2 are the same or different.

La présente invention concerne un procédé de préparation de liposomes consistant à: (I) mélanger au moins un lipide de formation de liposome, un solvant organique hydrosoluble et un milieu aqueux Y afin d'obtenir un gel ou un liquide contenant des particules de gel sans faire appel à la sonication; puis (ii) (a) mélanger le gel ou le liquide contenant des particules de gel avec un milieu aqueux Z1 afin d'obtenir directement des liposomes; (b) (i) mélanger le gel ou le liquide contenant des particules de gel avec un milieu aqueux Z1 afin d'obtenir un caillé ou une substance avec aspect de caillé avec un milieu aqueux Z2 afin d'obtenir directement des liposomes; ou (c) (i) refroidir le gel ou le liquide contenant les particules de gel afin d'obtenir une substance cireuse; et (ii) mélanger la substance cireuse avec le milieu aqueux Z1 afin d'obtenir directement des liposomes; les milieux aqueux Y, Z1 et Z2 étant identiques ou différents.

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