Efficient method for nuclear reprogramming

C - Chemistry – Metallurgy – 12 – N

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C12N 5/071 (2010.01) C12N 15/00 (2006.01) C12N 15/11 (2006.01) C12N 15/85 (2006.01)

Patent

CA 2658463

A method of preparing induced pluripotent stem cells, comprising a nuclear reprogramming step using a nuclear reprogramming factor in the presence of miRNA, wherein said miRNA has a property of providing a higher nuclear reprogramming efficiency in the presence of said miRNA than in the absence thereof.

L'invention concerne un procédé pour produire efficacement des cellules souches pluripotentes artificielles qui comprend l'étape consistant à procéder à une initialisation de noyaux au moyen d'un facteur d'initialisation de noyaux en présence d'un miARN, le miARN ayant les caractéristiques suivantes : (a) il est exprimé dans les cellules souches embryonnaires à un plus haut niveau que dans les cellules somatiques ; et (b) une efficacité d'initialisation de noyaux plus élevée a été établie en présence du miARN qu'en son absence.

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